ACTH 1-39 Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example ACTH 1-39 Titration Schedule
| Parameter | Details |
|---|---|
| Typical Dosage (Research Use) | 0.3 mg/kg/day |
| Route of Administration | Subcutaneous (SC) or Intramuscular (IM) |
| Frequency | Once daily |
| Cycle Length | Up to 6 hours (Infusion) to several weeks (Repository Injection) |
| Half‑life | 10–15 minutes |
| Reconstitution | For infusion: 0.25 mg in dextrose 5% in water or saline 0.9% (0.04 mg/hour rate) |
Possible vial strengths:
What Is It?
ACTH 1-39
Full-length adrenocorticotropic hormone.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix ACTH 1-39
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
ACTH 1-39 Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
The calculator's general model is based on smaller peptides, but the full sequence is different. The C-terminal region (residues 25-39) of human ACTH (1-39) doesn't change the initial binding, but it slows down the rate of degradation of the molecule. In a clinical study, the duration of response was up to 12 hours, while the shorter 1-24 fragment was active for only one hour.
While the calculator expects a quick dissolve, ACTH (1-39) is only soluble in water up to about 1 mg/mL. If your calculator suggests a high concentration that greatly exceeds 1 mg/mL, a standard aqueous solution may not achieve it. For higher concentrations, alternative solvents may be required.
The calculator's safe window may be too long for this peptide. To prevent aggregation and retain full potency, the reconstituted solution should be stored at 4°C for only 2-7 days. The long-term default will lead to degradation and inaccurate dosing. For long-term storage, you must aliquot and freeze the solution at -20°C.
The calculator only sees a mass, but ACTH (1-39) is a master hormone that strongly stimulates the adrenal glands to produce cortisol. This hormone has potent systemic and neuroprotective effects, but a dose that is even slightly too high will produce unwanted adrenal stress that your tool cannot account for.
Even when stored correctly, your peptide could be losing potency. ACTH contains an asparagine (Asn) residue that is prone to deamidation, a chemical alteration that occurs naturally in solution, particularly at neutral to alkaline pH. This is a built-in instability factor that will degrade your peptide over time.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.