ARA-290 Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
⚠ Use acetic acid — not bacteriostatic water
ARA-290 is insoluble or unstable in standard BAC water. Manufacturer protocols require reconstitution in dilute acetic acid (0.1%) — or, for IGF-1 variants, 50 mM acetic acid. Volumes shown below refer to acetic acid, not water.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example ARA-290 Titration Schedule
| Protocol Item | Guidance |
|---|---|
| Dose | 4–8 mg per dose |
| Cycling | Daily for 30-day cycles (or shorter), with a 30-day minimum break between cycles |
Possible vial strengths:
What Is It?
ARA-290
Erythropoietin-derived peptide investigated for neuropathy.
Acetic Acid (0.1%)
A dilute solution of acetic acid (typically 0.1% for most peptides, or 50 mM for IGF-1 variants) used to reconstitute peptides that are insoluble or unstable in standard bacteriostatic water. ARA-290 requires an acidic carrier to dissolve fully and remain stable — plain BAC water will not work and may damage the peptide.
How To Mix ARA-290
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw Acetic AcidDraw the selected amount of 0.1% acetic acid.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
ARA-290 Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
Convert mcg to mg by dividing by 1000. For example, 500 mcg = 0.5 mg; 2 mg stays as 2. Enter that number in the “Desired dose” field. The calculator works with decimals. Always verify you entered mg – entering 500 mg instead of 0.5 mg would be 1000× too high and likely show “Poor” or impossible volumes.
With 10 mg in 1 mL, concentration = 10 mg/mL. For 0.5 mg, volume = 0.05 mL = 5 units on a 100‑unit syringe. 5 units is above the calculator’s “poor” threshold (≥4 units), but might be “Usable” if it falls on a whole unit. However, 5 units is a very small volume – relative error can be high. The calculator may mark it as “Usable” or “Good” if it’s a multiple of 5. To improve accuracy, use a larger BAC volume: e.g., 10 mg in 2 mL = 5 mg/mL, 0.5 mg = 0.1 mL = 10 units (easier to measure). The calculator’s color coding helps you identify such cleaner options.
For a 1 mg dose on a 100‑unit syringe with 2 mL BAC water:
5 mg vial → 2.5 mg/mL → 1 mg = 0.4 mL = 40 units (×5) → Best
6 mg vial → 3 mg/mL → 1 mg = 0.333 mL = 33.3 units (not whole) → Poor
10 mg vial → 5 mg/mL → 1 mg = 0.2 mL = 20 units (×5) → Best
12 mg vial → 6 mg/mL → 1 mg = 0.1667 mL = 16.67 units → Usable/Poor
The calculator highlights green for Best matches. Choose a vial that, combined with your BAC volume, gives a round number of units (multiples of 5) for your target dose.
5 mg vial → 2.5 mg/mL → 1 mg = 0.4 mL = 40 units (×5) → Best
6 mg vial → 3 mg/mL → 1 mg = 0.333 mL = 33.3 units (not whole) → Poor
10 mg vial → 5 mg/mL → 1 mg = 0.2 mL = 20 units (×5) → Best
12 mg vial → 6 mg/mL → 1 mg = 0.1667 mL = 16.67 units → Usable/Poor
The calculator highlights green for Best matches. Choose a vial that, combined with your BAC volume, gives a round number of units (multiples of 5) for your target dose.
No, the calculator only performs mathematical dilution calculations. It does not distinguish between bacteriostatic water, sterile water, or saline. However, for multi‑use vials (more than one dose), bacteriostatic water is strongly recommended to prevent bacterial growth. The calculator’s BAC volume options (1,2,3,5 mL) work the same regardless of what liquid you actually use – the math for concentration and units is identical.
Yes, you do not need to remix the vial for each dose. Once you reconstitute a vial with a chosen BAC volume, keep the same vial strength and BAC volume in the calculator. Simply change the “Desired dose” field to your new mg amount – the calculator instantly updates the required units. Example: 10 mg vial + 2 mL BAC → 5 mg/mL.
0.5 mg → 0.1 mL = 10 units
1.0 mg → 0.2 mL = 20 units
2.0 mg → 0.4 mL = 40 units
All are multiples of 10, so accuracy remains high. The calculator’s “Doses per vial” display will update based on your current dose.
0.5 mg → 0.1 mL = 10 units
1.0 mg → 0.2 mL = 20 units
2.0 mg → 0.4 mL = 40 units
All are multiples of 10, so accuracy remains high. The calculator’s “Doses per vial” display will update based on your current dose.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.