GHK Basic Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example GHK Basic Titration Schedule
| Protocol Item | Guidance |
|---|---|
| Dose | 1–5 mg per dose |
| Cycling | Daily, for 6 weeks, with a 30-day minimum cycle break between |
| Annual Frequency | Can be cycled 3–4× per year |
Possible vial strengths:
What Is It?
GHK Basic
Tripeptide implicated in tissue remodeling.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix GHK Basic
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
GHK Basic Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
Yes, but you need to increase the BAC water volume to 5 mL for a 5 mg vial to get a concentration of 1 mg/mL. Then 0.05 mg = 0.05 mL = 5 units – this is the minimum measurable volume. The calculator will mark it as “Usable” or “Good” (if it lands on a multiple of 5). If you try a smaller BAC volume (1 mL), you get 1 unit – “Poor”, because the error is too high.
The calculator lets you select larger BAC volumes (e.g., 3 mL or 5 mL), which lowers the peptide concentration. For a 10 mg vial: with 1 mL BAC → 10 mg/mL; with 5 mL BAC → 2 mg/mL. For a 1 mg dose: first option gives 0.1 mL (10 units), second gives 0.5 mL (50 units). The larger volume may be less painful, even if it’s not a perfect multiple of 5. The calculator shows both options with color coding – choose the one that gives a larger draw volume, even if it is “Good” instead of “Best”.
Yes, the dilution math is identical because you enter the mass in mg regardless of whether it is pure GHK or GHK‑Cu. However, GHK‑Cu is blue and may precipitate at high concentrations (e.g., 50 mg/mL). The calculator does not account for this. For GHK‑Cu, always use a larger BAC volume (3–5 mL), even if the calculator shows “Best” for 1 mL.
Yes, the calculator only does concentration math – it does not care what liquid you use. But if you plan to use the vial more than once, non‑bacteriostatic water will allow bacterial growth. The calculator does not warn about this. For multi‑dose vials, you must use bacteriostatic water (0.9% benzyl alcohol). For a single, immediate injection, any sterile water or saline works.
No, the calculator does not know about pharmacokinetics. It simply tells you how many units to draw for a given mg dose. However, because GHK clears quickly, researchers often inject daily or twice daily. The calculator helps you draw the same dose every time from the same vial – just enter the same mg number in the “Desired dose” field. Pay attention to the “Doses per vial” display to know how many days your vial will last.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.