SS-31 and BAC Water Mix

SS-31 Reconstitution Calculator

Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.

What amount do you need?

Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.

Syringe size:
Possible vial strengths:
Best Match Good Match Usable Harder to Measure

Example SS-31 Titration Schedule

Protocol ItemGuidance
Dose2–5 mg per dose
FrequencyDaily
Cycle10–30 days on, then a minimum 30-day cycle break between cycles
Possible vial strengths:

What Is It?

SS-31

Mitochondria-targeted antioxidant tetrapeptide.

Bacteriostatic Water

Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.

How To Mix SS-31

1
Clean

Use alcohol swabs to clean the tops of both vials.

2
Draw BAC Water

Draw the selected amount of bacteriostatic water.

3
Inject Slowly

Add the liquid slowly down the side of the vial.

4
Swirl Gently

Do not shake. Swirl gently until dissolved.

5
Store Properly

Store as directed and protect from heat and light.

Best Practices & Common Mistakes

Best Practices

  • Use sterile technique.
  • Protect from light and heat.
  • Store refrigerated when appropriate.
  • Use clean syringe-unit math before measuring.

Common Mistakes

  • Confusing milligrams with milliliters.
  • Choosing an option with awkward decimal units.
  • Using too little liquid for very small measurements.
  • Shaking the vial aggressively.

SS-31 Storage & Handling

Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.

Frequently Asked Questions

Yes, but a clinical protocol uses 6 mL of bacteriostatic water for a 300 mg vial. The peptide is water-soluble up to 100 mg/mL.
The elimination half-life is short—2–3 hours after subcutaneous injection. This means your calculator’s “single daily dose” may need adjustment. Some research splits the daily dose into 2 injections to maintain levels.
The information is conflicting. Your calculator likes neat numbers, but a specific protocol uses 3 mL of BAC for a 30 mg vial (~10 mg/mL). This makes 1 unit = 100 mcg.
Yes. The approved commercial version is the hydrochloride (HCl) salt, while research-grade is often TFA. Although differences are small, the molecular weight may be reported as 639.79 or 749.2.
Once mixed, it is stable at 2–8°C for only 2 weeks. According to the calculator, discard any remaining solution after 4 weeks.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.
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