FOXO4-DRI Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example FOXO4-DRI Titration Schedule
| Protocol Item | Guidance |
|---|---|
| Dose | 1–5 mg per dose |
| Cycling | Daily, for 30-day cycles (or shorter), with a 30-day minimum cycle break between |
| Cycle Frequency | Based on the individual response |
Possible vial strengths:
What Is It?
FOXO4-DRI
Senolytic peptide studied for cellular aging.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix FOXO4-DRI
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
FOXO4-DRI Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
Divide mcg by 1000. For example, 50 mcg = 0.05 mg; 100 mcg = 0.10 mg; 200 mcg = 0.20 mg. Enter the mg value in the “Desired dose” field. Be very careful: a common research dose of 100 mcg is entered as 0.1, not 100. The calculator works with decimals.
With 10 mg in 1 mL, concentration = 10 mg/mL → 0.1 mg = 0.01 mL = 1 unit on a 100‑unit syringe. Any volume below 4 units is marked “Poor” because it is impossible to measure accurately. To fix this, increase BAC volume or use a smaller vial:
10 mg + 5 mL BAC → 2 mg/mL → 0.1 mg = 0.05 mL = 5 units (now “Usable”)
5 mg + 5 mL BAC → 1 mg/mL → 0.1 mg = 0.1 mL = 10 units (→ Best)
The color coding guides you to the most accurate option.
10 mg + 5 mL BAC → 2 mg/mL → 0.1 mg = 0.05 mL = 5 units (now “Usable”)
5 mg + 5 mL BAC → 1 mg/mL → 0.1 mg = 0.1 mL = 10 units (→ Best)
The color coding guides you to the most accurate option.
For a 0.1 mg dose on a 100‑unit syringe:
5 mg vial + 5 mL BAC → 1 mg/mL → 0.1 mg = 0.1 mL = 10 units (×5 → Best)
6 mg vial + 3 mL BAC → 2 mg/mL → 0.1 mg = 0.05 mL = 5 units (×5 → Best)
10 mg vial + 5 mL BAC → 2 mg/mL → 0.1 mg = 0.05 mL = 5 units (×5 → Best)
Avoid 1 mL BAC – it creates concentrations that are far too high for these small doses. The calculator highlights green (Best) for multiples of 5 units.
5 mg vial + 5 mL BAC → 1 mg/mL → 0.1 mg = 0.1 mL = 10 units (×5 → Best)
6 mg vial + 3 mL BAC → 2 mg/mL → 0.1 mg = 0.05 mL = 5 units (×5 → Best)
10 mg vial + 5 mL BAC → 2 mg/mL → 0.1 mg = 0.05 mL = 5 units (×5 → Best)
Avoid 1 mL BAC – it creates concentrations that are far too high for these small doses. The calculator highlights green (Best) for multiples of 5 units.
No, the calculator assumes complete solubility in bacteriostatic water at the concentrations generated by 1,2,3,5 mL BAC. However, FOXO4‑DRI often requires a small amount of DMSO or acetic acid to dissolve properly before adding BAC water. The calculator does not warn about solubility limits. If you reconstitute with only BAC water and see cloudiness or particles, do not inject. Research the correct reconstitution protocol for FOXO4‑DRI before using the calculator’s suggested volumes.
Yes, provided you have successfully dissolved it with the appropriate solvent and store the vial refrigerated (2–8°C). Bacteriostatic water, if used, preserves the solution for about 28 days. For a 10‑day cycle at 0.1 mg/day, total needed = 1 mg. A 5 mg vial is more than enough. The calculator’s “Doses per vial” display helps you plan: 5 mg / 0.1 mg = 50 doses. However, FOXO4‑DRI may degrade or precipitate over time; inspect the solution before each injection. If it becomes cloudy, discard and prepare a fresh vial.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.