BAM-15 Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example BAM-15 Titration Schedule
📋 BAM-15 Research Protocols: Preclinical & Administration Summary
The table below collates various laboratory animal protocols from published studies. Human-equivalent doses would require careful scaling calculations and are not recommended.
Context / Study Use Reported Dosage / Concentration Route / Formulation Frequency / Duration
General Mouse Study (Diet-Induced Obesity, Insulin Resistance) 0.1% and 0.2% (w/w) Admixed in diet Daily for up to 4 weeks
Aging/Sarcopenia Mouse Study 0.033% (mg/g) Standard chow admixture Daily for 8 weeks
Kidney Injury/Sepsis Mouse Study 1 mg/kg to 5 mg/kg Intraperitoneal (i.p.) injection Single dose or 3h before LPS
Inflammation Mouse Study 1 mg/kg Intraperitoneal (i.p.) injection 3h prior to LPS injection
Stroke/Cognitive Function Mouse Study 2.5 mg/kg Intraperitoneal (i.p.) injection Weekly or as detailed in protocol
Energy Expenditure Mouse Study 5, 10, 20 mg/kg (single dose) Intraperitoneal (i.p.) injection Single dose to measure metabolic changes
Liver & Metabolic Mouse Study 0.1% to 0.2% (w/w) Admixed in diet Daily feeding
Anti-Cancer Research 50 ng/mL (intratumoral) Targeted delivery (e.g., TCV-BAM15) As per protocol
Mitochondrial Function (in vitro) 100 nM to 50 µM Cell culture media Acute treatment (16h in some protocols)
The table below collates various laboratory animal protocols from published studies. Human-equivalent doses would require careful scaling calculations and are not recommended.
Context / Study Use Reported Dosage / Concentration Route / Formulation Frequency / Duration
General Mouse Study (Diet-Induced Obesity, Insulin Resistance) 0.1% and 0.2% (w/w) Admixed in diet Daily for up to 4 weeks
Aging/Sarcopenia Mouse Study 0.033% (mg/g) Standard chow admixture Daily for 8 weeks
Kidney Injury/Sepsis Mouse Study 1 mg/kg to 5 mg/kg Intraperitoneal (i.p.) injection Single dose or 3h before LPS
Inflammation Mouse Study 1 mg/kg Intraperitoneal (i.p.) injection 3h prior to LPS injection
Stroke/Cognitive Function Mouse Study 2.5 mg/kg Intraperitoneal (i.p.) injection Weekly or as detailed in protocol
Energy Expenditure Mouse Study 5, 10, 20 mg/kg (single dose) Intraperitoneal (i.p.) injection Single dose to measure metabolic changes
Liver & Metabolic Mouse Study 0.1% to 0.2% (w/w) Admixed in diet Daily feeding
Anti-Cancer Research 50 ng/mL (intratumoral) Targeted delivery (e.g., TCV-BAM15) As per protocol
Mitochondrial Function (in vitro) 100 nM to 50 µM Cell culture media Acute treatment (16h in some protocols)
Possible vial strengths:
What Is It?
BAM-15
Mitochondrial uncoupler research compound.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix BAM-15
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
BAM-15 Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
Critical. BAM‑15 is a small molecule, not a peptide. It has a molecular weight of 340.3 g/mol and a distinctive chemical structure — an oxadiazolo-pyrazine diamine. Unlike peptides, it cannot be reconstituted with bacteriostatic water. Your calculator's peptide‑focused logic does not apply.
Your calculator will suggest BAC water as the solvent, but this will fail. BAM‑15 is insoluble in water and ethanol at up to 1 mg/mL. Reconstitution of the lyophilised powder requires a two‑step process: first dissolve in anhydrous DMSO (up to 68 mg/mL) , then carefully dilute this stock solution into your experimental media. For animal injections, a standard vehicle is 10% DMSO, 40% PEG300, 5% Tween‑80, and 45% saline to achieve a clear solution at ≥2.5 mg/mL.
No. The plasma half‑life of BAM‑15 in mice is 1.7 hours (~1.5 hours). Pre‑clinical oral studies were conducted by mixing the compound into the diet at a fixed concentration (e.g., 0.1–0.2% w/w) for ad libitum feeding. A single daily injection would likely be insufficient to maintain effective levels. You need to consider multiple daily doses or a continuous administration method.
BAM‑15 is a potent mitochondrial protonophore (an anionic uncoupler) with a published EC₅₀ of 1.4 µM. One of its distinct characteristics is that it continues to drive high‑level, uncoupled respiration across a wide concentration range (3–100 µM) without causing collapse — and it does this without depolarising the plasma membrane. It is also about sevenfold more potent than the classic uncoupler 2,4‑dinitrophenol (DNP) in stimulating oxygen consumption. The calculator's standard linear scaling may be misleading.
No. Oral dosing in diet‑induced obesity studies is typically administered via feed admixture, with effective doses cited as 0.10–0.15% (w/w) in the diet, which consistently prevented fat accumulation. A fixed mass (e.g., 5 mg) without knowing the food intake per animal is not an equivalent dosing strategy. Your calculator must include a way to convert mg into a percentage of the total feed mass.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.