NSI-189 Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
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Example NSI-189 Titration Schedule
| Parameter | Details |
|---|---|
| Typical Dosage (Clinical Trial) | 40 mg, administered once, twice, or three times daily |
| Route of Administration | Oral (tablet/capsule) |
| Frequency | Once daily (QD) to three times daily (TID) |
| Cycle Length | 28 days treatment, with 56 days follow‑up |
| Half‑life | 17.4–20.5 hours; steady state achieved after 96–120 hours |
| Important Notes | Stimulates hippocampal neurogenesis; mechanistic target unknown；R |
| Most Common Adverse Events | Headache, dizziness, somnolence (drowsiness) |
| Legal Status | Investigational (Phase 2 clinical trials); Not FDA‑approved |
Possible vial strengths:
What Is It?
NSI-189
Neurogenic research compound.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix NSI-189
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
NSI-189 Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
No. NSI-189 is a small molecule, not a peptide, and is insoluble in water (<1 mg/mL). For research, first dissolve it in DMSO (up to 60-73 mg/mL), then dilute to the final concentration using a multi‑component vehicle (e.g., saline + PEG300 + Tween‑80) for injection.
Yes. The compound has a mean half‑life of 17.4‑20.5 h, reaching steady‑state after 4‑5 days. This supports once‑daily oral dosing (or twice‑daily for some protocols, e.g., 40 mg twice daily).
For storage of the powder, it is stable for 3 years at –20 °C and 2 years at 4 °C. For a solution, store at –20 °C and use within 3 months to prevent loss of potency. Refrigeration alone (4 °C) is not sufficient for long‑term storage.
The calculator cannot capture this. NSI-189 works by stimulating neurogenesis (new neuron growth) via up‑regulation of BDNF, SCF, VEGF, and GDNF. This transcriptional mechanism requires days to weeks of sustained exposure before biological effects are observed, and a short treatment course can produce effects lasting for months.
Human clinical doses range from 40 mg to 80 mg/day. In animal models, effective oral doses are 10‑30 mg/kg/day. These are milligram‑scale doses, not micrograms. Your calculator will massively under‑dose if you rely on peptide‑scale assumptions.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.