Tesamorelin Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example Tesamorelin Titration Schedule
| Protocol Item | Guidance |
|---|---|
| Dose | 500–2000 mcg per dose |
| Frequency | 1–7× weekly |
| Cycle | 8–12 weeks on, followed by 4–8 weeks off |
| Timing / Food Notes | Carbohydrates and fatty acids may blunt growth hormone release. The source notes it is best taken 30 minutes before eating or 2 hours after eating. |
| Additional Note | The source notes multiple injections in a day may be used to increase GH secretion and mentions bodybuilding use. |
Possible vial strengths:
What Is It?
Tesamorelin
Stabilised GHRH analogue.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix Tesamorelin
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
Tesamorelin Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
This is the most critical question for your calculator. Tesamorelin is not a "sustained-release" peptide. Even though it's cleared quickly (half-life range of 8-38 minutes), its purpose is to create a sharp pulse of GH release, which has downstream effects lasting over 24 hours. Your calculator works correctly here; the once-daily protocol is accurate, even if the molecule itself is short-lived.
This is a major contradiction your calculator can't resolve. The FDA label for the first approved version states to discard the reconstituted solution immediately and not freeze or refrigerate. However, a new formulation (EGRIFTA WR) is explicitly stable at room temperature (20°C to 25°C) even after mixing. You must follow your specific vial's label; the "refrigerate all peptides" rule does not apply to most Tesamorelin products.
This is a salt‑vs‑free‑base correction. The pharmaceutical product EGRIFTA SV contains 2 mg of tesamorelin acetate in the vial, but it delivers 1.4 mg of the free base peptide from that vial. Your calculator treats all mass as pure active peptide. If you enter "2 mg," you are overestimating the dose by about 30%. For accuracy, you must manually adjust the entered mass to reflect the free‑base equivalent stated on your Certificate of Analysis.
Yes, and your calculator can't account for this. The absolute bioavailability of subcutaneously injected tesamorelin is less than 4% in healthy adults. This means that only a tiny fraction of the mass you dissolve and draw up actually reaches the systemic circulation. A "2 mg" dose therefore only delivers about 80 mcg of active peptide to the bloodstream.
The approved formulation is not a multi‑dose vial. The original product is a single‑use vial that should be administered immediately after mixing. For research‑grade powders, the stability of the reconstituted solution may be unknown, and suppliers often recommend using it within 24 hours. The chemical degradation is highly formulation‑specific; BAC water does not magically preserve it for weeks.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.