HMG Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example HMG Titration Schedule
| Parameter | Details |
|---|---|
| Typical Dosage (Female) | 75–225 IU/day (starting dose commonly 75–150 IU) [7†L28-L29]. Max daily: 450 IU (treatment >20 days not recommended) [9†L42-L43]. |
| Typical Dosage (Male) | For spermatogenesis: 75–150 IU three times weekly [7†L47-L48]. |
| Route | Subcutaneous (SC) or Intramuscular (IM) [4†L44-L45][5†L5-L6]. |
| Frequency (Female) | Once daily, typically for 5–12 days (adjusted based on response) [7†L30-L31]. |
| Frequency (Male) | Combined therapy with hCG for at least 4 months [5†L20-L21]. |
| Cycle Length (Female) | Treatment until adequate ovarian follicular development (then trigger with hCG 5,000–10,000 IU) [4†L37-L38]. |
| Half‑life (sc) | 51 hours [4†L20-L22]. |
Possible vial strengths:
75IU, 150IU
What Is It?
HMG
Human menopausal gonadotropin reference compound.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix HMG
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
HMG Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
Unlike synthetic peptides, HMG’s bioactivity is measured in International Units (IU), not by mass. If you know your vial’s specific activity (e.g., ~500 IU/mg), you can convert your desired IU dose into mg first. For a 75 IU vial, the peptide mass is only around 0.15 mg.
A common strategy is to use 3.0 mL of BAC water for a 75 IU vial, creating a concentration of 25 IU per 0.1 mL (10 units on a U-100 syringe). This makes common doses like 75 IU (0.3 mL) or 150 IU (0.6 mL) easy to measure.
For HMG, the correct stability window is much shorter. Once mixed, the solution should be stored at 4°C for only 2–7 days. It can be frozen (–18°C) for future use, but must be split into single-use aliquots to avoid repeated freeze-thaw cycles.
The FSH activity in HMG has a very long elimination half-life (around 41–45 hours). Your calculator’s linear model assumes the dose is fully cleared within 24 hours, but HMG’s effect is sustained, supporting an injection schedule of 2–3 times per week.
HMG is a complex mixture containing FSH, LH, and trace amounts of hCG as a stabilizer. Unlike pure peptides that target a single receptor, HMG triggers multiple pathways. Because it contains hCG, some experts classify it as a “dual-action” compound where LH activity is prolonged compared to natural LH.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.