HCG Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example HCG Titration Schedule
| Parameter | Details |
|---|---|
| Ovulation Induction | 5,000–10,000 units IM once, 1 day after last FSH dose; OR r-HCG 250 mcg SC once, 1 day after last FSH dose |
| Hypogonadism, Male | 500–1,000 units IM 3x weekly ×3 weeks, then 2x weekly ×3 weeks; OR 4,000 units IM 3x weekly ×6–9 mos, then 2,000 units IM 3x weekly ×3 mos |
| Cryptorchidism, Prepubertal | 4,000 units IM 3x weekly ×3 weeks; OR 5,000 units IM every other day ×4 injections; OR 500–1,000 units IM ×15 injections over 6 weeks; OR 500 units IM 3x weekly ×4–6 weeks. Typical age: 4–9 years |
| Male TRT Adjuvant (Off‑Label) | 500–1,000 units SC 2–3x weekly, adjusted to maintain total testosterone 350–900 ng/dL |
| Spermatogenesis Induction | 1,500–3,000 units SC twice weekly (with FSH) until testosterone normalizes |
| Simeons Weight Loss Protocol (Experimental/Refuted) | 125 IU IM daily, 6 days/week, ×40 total injections, with 500‑calorie/day diet |
| Route | Intramuscular (IM) – standard; Subcutaneous (SC) – with recombinant HCG |
| Reconstitution | For 10,000 IU vial: use 1 mL diluent → 10,000 IU/mL; or 10 mL diluent → 1,000 IU/mL |
| Half‑life | ~24–36 hours (urinary HCG); recombinant HCG ~29 hours (IV) |
Possible vial strengths:
5000IU, 10000IU
What Is It?
HCG
Human chorionic gonadotropin reference compound.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix HCG
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
HCG Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
Your calculator assumes mass-based dosing. HCG is a glycoprotein, and its activity is defined biologically: 1 IU is the potency of 0.125 μg (or 125 pg) of the International Standard. To use the calculator, you can't just enter a weight; you first need to know the specific activity of your batch (e.g., 5,000 IU/mg). You would then convert your desired IU dose into mg before using the calculator—a step your tool was not designed for.
Both are options, but this isn't trivial. The included 1 mL vial provides a highly concentrated solution (e.g., 10,000 IU/mL). While fine for high single doses, a 200 IU dose would be just 0.02 mL (2 units)—almost impossible to measure accurately. The standard user practice is to use a larger volume (e.g., 2–10 mL) of a separate 30 mL vial of BAC water to create a much more manageable injection volume (e.g., 200 IU = 0.2 mL or 20 units).
Your calculator's default would be wrong. The specific stability data for HCG states that the reconstituted solution should be stored at 4 °C for only 2‑7 days. For any longer storage, the solution must be aliquoted and frozen at –18 °C.
Yes, this is a common practice for HCG. Once refrigerated, the peptide inside a sealed syringe is just as stable as in the original vial. Some users also store these pre‑loaded syringes in a freezer for long‑term preservation, but you must allow them to thaw completely before injection to avoid cold-related discomfort.
This is where sources conflict. Some manufacturers state 4–6 weeks, while research product data sheets limit it to 2‑7 days. Given this ambiguity, the safest practice is to follow the shorter 2‑7 day window. If you need to use the same vial for longer, freeze single‑use aliquots immediately after mixing.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.