Follistatin-315 Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example Follistatin-315 Titration Schedule
| Protocol Item | Guidance |
|---|---|
| Dose | 100–300 mcg per dose |
| Cycling | 8 weeks on, 4–8 weeks off |
| Administration Note | Some references theorize that localized administration (e.g., near the target muscle) may be a best approach. |
Possible vial strengths:
What Is It?
Follistatin-315
Myostatin-binding follistatin isoform.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix Follistatin-315
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
Follistatin-315 Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
Divide mcg by 1000 to get mg. For example, 100 mcg = 0.1 mg; 300 mcg = 0.3 mg; 500 mcg = 0.5 mg. Enter the mg value in the “Desired dose” field. The calculator works with decimals. Be careful not to enter the mcg number as mg – 500 mg instead of 0.5 mg would be 1000× too high and likely impossible to draw.
With 10 mg in 1 mL, concentration = 10 mg/mL → 0.2 mg = 0.02 mL = 2 units on a 100‑unit syringe. Any volume below 4 units is marked “Poor” because it is extremely difficult to measure accurately. To fix this, use a larger BAC volume: 10 mg + 5 mL BAC → 2 mg/mL → 0.2 mg = 0.1 mL = 10 units → “Best”. Or use a smaller vial: 5 mg + 5 mL BAC → 1 mg/mL → 0.2 mg = 0.2 mL = 20 units → “Best”. The calculator’s color coding guides you.
For a 0.3 mg dose on a 100‑unit syringe:
5 mg vial + 5 mL BAC → 1 mg/mL → 0.3 mg = 0.3 mL = 30 units (×5 → Best)
6 mg vial + 3 mL BAC → 2 mg/mL → 0.3 mg = 0.15 mL = 15 units (×5 → Best)
10 mg vial + 5 mL BAC → 2 mg/mL → 0.3 mg = 0.15 mL = 15 units (×5 → Best)
Avoid 1 mL BAC with small doses. The calculator highlights green for the most accurate draws.
5 mg vial + 5 mL BAC → 1 mg/mL → 0.3 mg = 0.3 mL = 30 units (×5 → Best)
6 mg vial + 3 mL BAC → 2 mg/mL → 0.3 mg = 0.15 mL = 15 units (×5 → Best)
10 mg vial + 5 mL BAC → 2 mg/mL → 0.3 mg = 0.15 mL = 15 units (×5 → Best)
Avoid 1 mL BAC with small doses. The calculator highlights green for the most accurate draws.
No, the calculator assumes complete solubility at the concentrations generated by the BAC volumes (1,2,3,5 mL). However, Follistatin‑315 can be difficult to dissolve, especially at high concentrations (e.g., 10 mg in 1 mL = 10 mg/mL). For reliable reconstitution, always use the largest BAC volume (5 mL) and let the peptide dissolve slowly, possibly with gentle swirling and refrigeration. If the solution remains cloudy, do not use it. The calculator does not warn about solubility limits – you must research the recommended concentration for your specific formulation.
Yes, if you reconstituted with bacteriostatic water and store the vial refrigerated (2–8°C). Bacteriostatic water preserves the solution for about 28 days. For a typical dose of 0.3 mg every other day, a 5 mg vial gives about 16 doses (5 / 0.3 = 16.6), which lasts about 32 days – slightly longer than the 28‑day recommendation. Consider using a 10 mg vial if you need more doses or split your reconstitution into multiple vials. The calculator’s “Doses per vial” display helps you plan. Always check for precipitation before each use.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.