Cotadutide Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example Cotadutide Titration Schedule
| Parameter | Details |
|---|---|
| Dosage | 50–600 µg once daily (titrate: 100 µg → 200 µg → 300 µg → 400 µg → 600 µg) |
| Route | Subcutaneous (SC) |
| Frequency | Once daily |
| Cycle Length | Up to 26 weeks (clinical trials) |
| Half‑life | ~10 hours |
| Reconstitution | DMSO → dilute in saline or PBS |
Possible vial strengths:
What Is It?
Cotadutide
GLP-1 / glucagon dual agonist.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix Cotadutide
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
Cotadutide Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
Yes. All clinical trials (Phase 1 – 2b) used once‑daily subcutaneous injection. The 13‑hour terminal half‑life supports daily dosing, not twice‑daily. Your calculator's daily schedule is correct.
Phase 2b used a 50 μg starting dose, increased by 50 μg every 4 weeks up to a maximum of 300 μg. Starting at a higher dose (e.g., 300 μg) causes severe nausea (∼92%). Your calculator's single‑dose output should not be used as the first injection.
The C‑terminal amide (‑NH₂ instead of ‑COOH) increases molecular weight by ∼0.98 Da. For a ∼3.3 kDa peptide, this is negligible (<0.03% error). The calculator's default mass handling is fine.
The clinical formulation uses 10 mM histidine buffer, pH 6.5, which maintains solubility and stability. BAC water (pH 5.5) is acidic enough to cause hydrolysis of the amide bond over time. For storage >1 week, use histidine buffer, not BAC water.
No. Cotadutide's unique benefit is activating glucagon receptors, which increases resting energy expenditure — something GLP‑1 alone does not do. The calculator treats all peptides as weight‑loss agents via appetite suppression, missing this distinct metabolic pathway.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.