AICAR Reconstitution Calculator
Enter the amount you want to measure. The vial buttons will highlight which vial strengths create cleaner syringe-unit measurements.
What amount do you need?
Type the target amount, then choose mg or mcg. Example: 2mg or 500mcg.
Syringe size:
Possible vial strengths:
Best Match
Good Match
Usable
Harder to Measure
Example AICAR Titration Schedule
| Research Context (as described) | Timeline | Dose | Notes |
|---|---|---|---|
| Skeletal muscle research (6.5-week protocol) | Weeks 1–4 | 500 mg/kg body weight (daily injection) | Front-loaded “high dose” phase |
| Skeletal muscle research (6.5-week protocol) | Weeks 4–6.5 | Lower dose (daily injection) | Article notes the dose is reduced, but does not specify the exact amount |
| Metabolic syndrome / type 2 diabetes example | Up to 13 weeks | 1 mg/kg per day (injections) | Presented as an example tied to blood sugar homeostasis research |
| Fat tissue metabolism example | 4–8 weeks | 0.5–1.0 g/kg per day (intraperitoneal injection) | Article cites 0.7 g/kg as an “optimal” value for this context |
Possible vial strengths:
What Is It?
AICAR
AMPK activator research compound.
Bacteriostatic Water
Sterile water containing a bacteriostatic preservative, commonly used when preparing multi-use research vials.
How To Mix AICAR
1
CleanUse alcohol swabs to clean the tops of both vials.
2
Draw BAC WaterDraw the selected amount of bacteriostatic water.
3
Inject SlowlyAdd the liquid slowly down the side of the vial.
4
Swirl GentlyDo not shake. Swirl gently until dissolved.
5
Store ProperlyStore as directed and protect from heat and light.
Best Practices & Common Mistakes
Best Practices
- Use sterile technique.
- Protect from light and heat.
- Store refrigerated when appropriate.
- Use clean syringe-unit math before measuring.
Common Mistakes
- Confusing milligrams with milliliters.
- Choosing an option with awkward decimal units.
- Using too little liquid for very small measurements.
- Shaking the vial aggressively.
AICAR Storage & Handling
Lyophilized Powder: −20°C (−4°F) for long-term storage (up to 24 months). Refrigeration 2–8°C (36–46°F) for short-term use (up to ~3 months). Original sealed vial in the freezer is safest.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Reconstituted Solution: 2–8°C (36–46°F), use within ~7–14 days. Keep sealed, avoid light, and do not repeat freeze-thaw cycles.
Frequently Asked Questions
You calculate your total mg dose first: body weight (kg) × desired mg/kg. For example, 80 kg × 50 mg/kg = 4000 mg (4 g). Enter that number (4000) in the “Desired dose” field. Then select your syringe size, vial strength, and BAC volume. The calculator will show how many units to draw. Note: AICAR requires very high mg doses, so you may need large vials (500 mg or more) or multiple injections.
AICAR doses are often hundreds or thousands of milligrams, but the calculator’s vial options only go up to 60 mg. For such high doses, you would need extremely large BAC volumes to keep the concentration low enough to fit in a syringe. The calculator is not designed for very high‑mass peptides. Consider using a different tool or manually calculate: for a 500 mg dose, a 60 mg vial would require 9 vials, which is impractical.
With the largest syringe (100 units = 1 mL) and the smallest BAC volume (1 mL), the maximum concentration is 50 mg/mL, so the max dose in one syringe is 50 mg (1 mL). If you need more, you must either reconstitute multiple vials and combine them, or use a larger syringe not listed. The calculator will warn you if your desired dose exceeds the syringe capacity by showing “Poor” for all options.
No, the calculator assumes complete solubility at the concentrations generated by the chosen BAC volumes (1,2,3,5 mL). For AICAR, solubility in bacteriostatic water is limited (~10–20 mg/mL). If the calculator suggests a concentration above ~15 mg/mL (e.g., 50 mg in 1 mL = 50 mg/mL), the peptide may not fully dissolve. You should manually ensure the concentration stays ≤15 mg/mL by adding more BAC water than the calculator’s preset options (e.g., use 5 mL for a 50 mg vial → 10 mg/mL).
Yes, once reconstituted, store the vial refrigerated. Each time you need a dose, enter the same desired mg amount in the calculator (using the same vial strength and BAC volume you initially used). The calculator will show the same units per dose. Keep track of how many doses you have drawn — the “Doses per vial” display helps. AICAR in solution may degrade faster than other peptides; consider using within 7–10 days.
Practical takeaway: If your real goal is weight or metabolic health, the most useful next step is discussing approved treatment options with a clinician rather than relying on an unapproved compound.
Important: This tool is for informational and research-reference purposes only. Not intended for human or veterinary use.